Journal:

Article Title: Human ?-Defensins HNPs-1, -2, and -3 in Renal Cell Carcinoma

doi:

Figure Lengend Snippet: RT-PCR analysis of HNP-1, -2, and -3 mRNA expression: 250 ng of cDNA from different cultured RCC lines, from the normal kidney cell line TK163, and PBMC of leukapheresis products were amplified for β-actin (A, control product, 317 bp) and HNP-1, -2, and -3 sequences (B, product 215 bp). Lane 1, Caki-2; lane 2, A-498; lane 3, ACHN; lane 4, 786-0; lane 5, 769-P; lane 6, LE 9211; lane 7, N43; lane 8, TW33; lane 9, PBMC; lane 10, TK163; lane 11, no cDNA.

Article Snippet: Cell Lines and Culture Conditions In this study, eight RCC lines were used that included five lines (A-498, Caki-2, 786-0, 769-P, ACHN) purchased from the American Type Culture Collection (ATCC; Manassas, VA), one line (LE 9211-RCC) obtained from Dr. B. van den Eynde (Ludwig Cancer Institute, Brussels, Belgium) and two lines (TW33, N43) were established from renal tumor tissues in our laboratory according to the previously described method.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Cell Culture, Amplification

Journal:

Article Title: Human ?-Defensins HNPs-1, -2, and -3 in Renal Cell Carcinoma

doi:

Figure Lengend Snippet: Influences of HNP-1, -2, and -3 on cell proliferation of different RCC lines. A: RCC lines were incubated with and without different concentrations of the isolated HNP-1, -2, and -3 peptide fraction. Dose-dependent proliferative responses were measured by [3H]-TdR incorporation given as mean counts per minute (cpm) or as formazan-dye production in viable cells (A, inset); the RCC lines A-498 and ACHN showed a dose-dependent, significant increase of proliferative activity after stimulation with HNP-1, -2, and -3 in comparison to background proliferation in cultures without HNP-1, -2, and -3. Similar results were obtained in six independent stimulation experiments as well as with the synthetic HNP-1 peptide. B: Inhibition of cell proliferation of the RCC line A-498 stimulated with 12.5 μg/ml of isolated HNP-1, -2, and -3 is shown by preincubation of HNP-1, -2, and -3 with 12.5 μg/ml of the mAb DEF3 in comparison to cultures of the cell line incubated with defensins only (control of autocrine proliferation: incubation with RPMI 1640 medium only) C: Evaluation of total cell number by fluorescence microscopy after culture of the RCC line A-498 for 40 hours both with 12.5 μg/ml of isolated HNP-1, -2, and -3 and without. A significant increase of cell nuclei indicating cell proliferation was observed after stimulation with defensins.

Article Snippet: Cell Lines and Culture Conditions In this study, eight RCC lines were used that included five lines (A-498, Caki-2, 786-0, 769-P, ACHN) purchased from the American Type Culture Collection (ATCC; Manassas, VA), one line (LE 9211-RCC) obtained from Dr. B. van den Eynde (Ludwig Cancer Institute, Brussels, Belgium) and two lines (TW33, N43) were established from renal tumor tissues in our laboratory according to the previously described method.

Techniques: Incubation, Isolation, Activity Assay, Inhibition, Fluorescence, Microscopy

Journal: The Cochrane Database of Systematic Reviews

Article Title: Angiotensin converting enzyme (ACE) inhibitors versus angiotensin receptor blockers for primary hypertension

doi: 10.1002/14651858.CD009096.pub2

Figure Lengend Snippet: All angiotensin converting enzyme (ACE) inhibitors versus all angiotensin receptor blockers (ARBs)

Article Snippet: One study compared telmisartan with ramipril, and was supported by a grant from Boehringer Ingelheim, along with grants from the Heart and Stroke Foundation of Ontario and the Canadian Institues of Health Research ( ONTARGET 2008 ).

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